Stabilization of a full-Length infectious cDNA clone for duck Tembusu virus by insertion of an intron.

Jiaqi Guo,Yu He,Xiaoli Wang,Bowen Jiang, Xiao Lin,Mingshu Wang,Renyong Jia,Dekang Zhu,Mafeng Liu,Xinxin Zhao,Qiao Yang,Ying Wu,Shun Chen,Anchun Cheng

Journal of Virological Methods（2020）

引用 12|浏览102

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摘要

•The plasmid of infectious cDNA clone containing intron is very stable without obviously prokaryotic toxicity.•Recombinant viruses were recovered by directly transfection of the plasmids into cells.•Recombinant viruses showed similar viral properties as the parent virus.

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关键词

Duck Tembusu virus,Reverse genetics system,Intron

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