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[Effect of Acupuncture on NLRP3 Inflammatory Corpuscle in Rats with Intracerebral Hemorrhage].

Zhongguo zhen jiu = Chinese acupuncture & moxibustion(2020)

Zhejiang Academy of TCM | Department of Acupuncture-Moxibustion

Cited 10|Views15
Abstract
OBJECTIVE:To observe the effect of acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage (ICH), and to explore the action mechanism of acupuncture on promoting the recovery of neural function in rats with ICH.METHODS:Forty SPF six-week-old male SD rats were randomly divided into a sham operation group, a model group, a non-acupoint group and an acupuncture group, 10 rats in each group. The rats in the model group, non-acupoint group and acupuncture group were intervened with autologous blood injection to prepare ICH model, while the rats in the sham operation group were only intervened with operation but not injection with autologous blood. About 3 hours after the establishment of the model, the rats in the acupuncture group were intervened with acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7), once every 12 hours, for 7 days; the rats in the non-acupoint group were intervened with acupuncture at the non-acupoint [parallel to the "Baihui" (GV 20), 1 cm next to the midline] on the affected side, and other treatment was the same as the acupuncture group. At the end of the intervention, the composite nerve function score of each group was evaluated; the histomorphology of the hemorrhage penumbra was observed by HE staining; the expression of NLRP3 inflammatory corpuscle in the brain was detected by immunohistochemistry; the relative protein expression levels of NLRP3, interleukin-1β (IL-1β) and interleukin-18 (IL-18) in brain were detected by the method of Western blot.RESULTS:Seven days into intervention, compared with the sham operation group, each item score and total score of composite nerve function in the model group were significantly reduced (P<0.01, P<0.05). There was edema and karyopyknosis in brain neuron as well as necrocytosis and inflammatory cell infiltration in the model group. Compared with the model group and the non-acupoint group, the total score of composite nerve function and the scores of symmetrical movement of limbs (LS) and proprioception of tentacles (VP) in the acupuncture group were increased (P<0.01, P<0.05), and the cell necrosis and inflammatory cell infiltration were relieved. Compared with the sham operation group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the model group were increased (P<0.01); compared with the model group and the non-acupoint group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the acupuncture group were reduced (P<0.01).CONCLUSION:Acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) could downregulate the expression of NLRP3, IL-1β and IL-18 in the brain tissue of ICH rats, inhibit the inflammatory response, and promote the recovery of neural function.
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要点】:研究探讨了针刺“百会”至“曲鬃”对脑出血大鼠NLRP3炎症小体的影响,揭示了针灸促进神经功能恢复的作用机制。

方法】:通过建立大鼠脑出血模型,并对模型组进行“百会”至“曲鬃”穴位针灸干预,采用HE染色观察脑组织形态学变化,免疫组化及Western blot检测NLRP3炎症小体在大脑中的表达及其相关蛋白表达水平。

实验】:实验使用40只SPF六级雄性SD大鼠,分为假手术组、模型组、非穴位组和针灸组,通过自体血注射制备脑出血模型,针灸组在模型建立后3小时开始进行“百会”至“曲鬃”穴位针灸,每12小时一次,共7天;非穴位组在模型建立后进行非穴位针灸,其他处理与针灸组相同。干预结束后,评价各组神经功能复合评分,观察脑出血半暗带组织形态学变化,检测脑组织中NLRP3炎症小体表达及NLRP3、白介素-1β(IL-1β)和白介素-18(IL-18)相对蛋白表达水平。结果显示,针灸组神经功能恢复较好,脑组织炎症反应减轻,NLRP3、IL-1β和IL-18表达降低。