Characterization of cells derived from inflamed intra-bony peri odontal defects

Methods: Cultures of ihPDLSCs were established from granulation tissue of intra-bony periodontal defects (n = 4). Expression of epitopes characteristic for mesenchymal (CD73, CD90, CD105, CD146, STRO-1), embryonic (SSEA-4) and hematopoietic (CD34, CD45) stem cells were analysed by flow cytometry. Neuronal, endothelial and osteoblastic differentiation was induced by respec tive media. Changes in cell morphology were observed microscopically. Matrix mineralization was visualized and quantified using Alizarin Red S staining. Gene expression of neurogenic (NEFL, NCAM1, ENO2, TUBB3), angiogenic (VEGFR1, VEGFR2, PECAM1, ANGPT2) and osteogenic (RUNX2, SP7, APL, BMP2, BGLAP, SPP1, IBSP) markers was assessed by qRT-PCR.