Efficient P ( 3 HB ) extraction from Burkholderia sacchari cells using safer , non-chlorinated solvents

A technique for poly-3-hydroxybutyrate (P(3HB)) extraction with safer, non-chlorinated solvents, was developed, aiming to attain high recovery yields and purities. A wide range of solvents was selected from the GlaxoSmithKline guide as sustainable industrial solvents and the solubility of P(3HB) on those solvents calculated using predictive equations from literature. Anisole, ∗ Corresponding author: Tel.: +351 21 8419137; Fax: +351 21 8419062. e-mail address: teresa.cesario@tecnico.ulisboa.pt + Current address: Politecnico di Milano, Dipartimento di Chimica Materiali e Ingegneria Chimica "Giulio Natta". Via Mancinelli, 7 20131 Milano MI