2 Characterization of Conformation of Therapeutic Antibody Aggregation with Optimized Hydrogen / Deuterium Exchange Mass Spectrometer

Herceptin and its pH stressed samples were quantified by measuring UV 280 absorption. Figure 4 is the overlay absorption spectra of four samples. Aggregations around 25% for pH 0.5, 6% for pH 1.5 and 0% for pH 2.5 were observed. Figure 3. a). Flexible three valve configuration for carrying out different experimental tasks simultaneously in the cooling chamber, b). NCS-3500RS with binary rapid separation micro flow pump with ternary loading pump