Utilization of Shrimp Shell Wastes to Produce Chitinase from Streptomyces macrosporeus InaCC A454

Chitinases (EC 3.2.1.14) hydrolyze chitin polymers into shorter oligomers and N-acetyl glucosamine monomers. Chitinis found in marineinvertebrates shell, particularly crustaceans, insects, mollusks, and fungal cell walls. Shrimp waste is very important source of chitin. This study aimed to determine actinomycetes ability from P. Belitung to produce chitinase with substrate contained chitin from shrimp waste. The chitinase enzyme was characterized including incubation time, the optimum pH, temperature and stability. The effect of metal ions as activators or inhibitors of the enzyme were measured using spectrophotometer at λ 584 nm. Actinomycetes strain was identifiedusing 16S rDNA sequences. Chitinase activity was qualitatively demonstrated by clear zone around the colonies in the medium containing 1% colloidal chitin. The result showed that the highest activity were incubation time of five days, temperature of 50oC and pH 8 by 0.0304 U/mL, 0.0312 U/mL and 0.0421 U/mL, respectively. At concentration of 1mM the enzyme were activated by divalent cations CaCl2, MnCl2, CuCl2 and monovalent cations KCl, NaCl. Molecular identification based on 16S rDNA sequences showed that actinomycetes isolate was Streptomyces macrosporeus. The strain was registered in the InaCC collection (no. A 454). © 2016 Indonesian Journal of Applied Chemistry. All rights reserved A R T I C L E I N F O Article history Received date : 31 August 2015 Revised date : 10 May 2016 Accepted date :10 June 2016 Available online at: http://kimia.lipi.go.id/inajac/index.php Kata kunci: Enzim kitinase, aktinomisetes, Streptomyces macrosporeus