Periprosthetic Through the Production Chronic Inflammation Characterized Measurement of Superoxide Anion by Synovial-Derived Macrophages

Periprosthetic macrophages were isolated from the synovium of primary and revision arthroplasty patients. Inflammatory activity was determined by the level of superoxide (0;) production de novo and in response to phorbol myristate acetate (PMA) stimulation. Nonstimulated primary arthroplasty-derived macrophages produced 2.54 k 2.04 pmoles of O;/minute/lOs cells. When identical reaction tubes were stimulated with PMA, 0; levels increased to 5.76 * 3.77 pmol of O;/minute/105 cells. Nonstimulated revision arthroplasty-derived macrophages produced 3.26 f 2.02 pmol of O;/minute/lOs cells during this ten-minute time period. When identical reaction tubes were stimulated with PMA, 0; levels increased to 3.98 f 2.52 pmol of O;/minute/lOs cells. The difference in the ratio of 0; production in response to