Transcriptomic Profiling Identifies Novel Transcripts, Isomorphs, And Noncoding Rnas In Paracoccidioides Brasiliensis

This paper describes a transcriptomic profiling of Paracoccidioides brasiliensis (Pb) performed with the aid of an RNA-seq-based approach, aimed at characterizing the general transcriptome in this human pathogenic fungus, responsible for paracoccidioidomycosis (PCM). Results confirm that similar to 75% of the genes currently annotated in the P. brasiliensis genome are, in fact, transcribed in vivo and that similar to 19% of them may display alternative isomorphs. Moreover, we identified 627 transcripts that do not match any gene currently mapped in the genome, represented by 114 coding transcripts (probably derived from previously unmapped proteincoding genes) and 513 noncoding RNAs (ncRNAs), including 203 long-noncoding RNAs (lncRNAs).