Study on the Methods for Synthesis of GM-CSF Conjugates with Alendronic Acid

— In order to develop a target drug we studied the process of conjugation of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and alendronic acid (ALN). The conjugation reactions were performed both in solution and in the solid phase. The results of the examination of the specific activity of the conjugated GM-CSF confirmed the preservation of biological properties of the protein. The experimental data obtained indicate a higher affinity of GM-CSF-ALN conjugates to hydroxyapatite, an analog of the bone mineral matrix, than of the intact protein.