Potentially Probioticlactobacillusstrains Derived From Food Intensify Crystallization Caused Byproteus Mirabilisin Urine

Proteus mirabilisis a common cause of infectious urolithiasis. The first stage in the formation of urinary stones is the crystallization of mineral salts in the urine induced by urease activity of this microorganism.Lactobacillusspp.are an important component of the human microbiota and in large quantities occur in foods. Regardless of their origin, those with probiotic properties are proposed as an alternative to antibiotic therapy in the treatment of urinary tract infections. The aim of the study was to check the effect of selectedLactobacillus plantarumandLactobacillus brevisstrains on crystallization caused byP. mirabilisin an in vitro experiment. It has been confirmed that selectedLactobacillusstrains have antibacterial properties and colonize the urinary tract epithelium. During 24-h incubation of bacterial cultures, containingP. mirabilisand individualLactobacillusstrains, in synthetic urine, bacterial viability (CFU/mL), pH, and crystallization were determined. Crystallization was assessed quantitatively and qualitatively using AAS and XRD techniques as well as phase-contrast microscopy. It has been shown that in the presence of selectedLactobacillusstrains, the culture pH increases faster, especially after 8 h of incubation, compared with the pureP. mirabilisculture. An increase in pH reduces the viability ofP. mirabilis; however, in the presence of some lactobacilli, the uropathogen grows more intensively. The presence ofLactobacillusalso affected crystallization by increasing its intensity, and the resulting crystals were larger in size. TestedL. plantarumandL. brevisstrains could therefore accelerate the formation of urinary stones and development of infection.