Control of Cell Migration Using Optogenetics

EPITHELIAL-TO MESENCHYMAL TRANSITION: Methods and Protocols（2021）

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摘要

Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion.

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关键词

Optogenetics, Rho GTPase, Cdc42, Polarity, Migration, Lamellipodium, Microscopy

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