Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase
CHEMICAL COMMUNICATIONS(2020)
Abstract
We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme-probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.
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Key words
terminal uridylyl transferase,responsive fluorescent nucleotides
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