Intra-exon motif correlations as a proxy measure for mean per-tile sequence quality data in RNA-Seq

The motif correlations as a function of both GC content parameters are much higher in the mRNA RNA-Seq sample (control) than in the other RNA-Seq samples that did undergo mRNA selection: both ribosomal depletion () and PolyA selection (, wild-type, and mutant). There is considerable degradation of similar correlations in the mutant samples from the dataset. This matches with the available mean tile data that has been gathered for these datasets. We observe that extremely low correlations are indicative of bias of technical origin, such as flowcell errors.