In Vitrostudies Of Antitumor Effect, Toxicity/Cytotoxicity And Skin Permeation/Retention Of A Green Fluorescence Pyrene-Based Dye For Pdt Application

Photosensitizers (PS) are compounds that can generate reactive oxygen species under irradiation of appropriate light and are widely used in photodynamic therapy (PDT). Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, PDT is also used to treat nonmelanoma skin cancer and can be a potential tool for melanoma, associated with other treatments. In this work, we evaluated the antitumor photoactivity of a new pyrene-based PS (TPPy) by using the murine melanoma cell line (B16F10). Thein vitropermeation/retention tests in porcine ear skin were also performed in order to evaluate the potential application of the PS for topical use in skin cancer. Moreover, to determine the toxicityin vivo, we used theGalleria mellonellaas an alternative animal model of study. The results showed that TPPy is a promising PS for application in PDT, with potential antitumor photoactivity (IC(50)6.5 mu mol L-1), absence of toxicity in theG. mellonellamodel at higher concentration (70.0 mmol L-1) and the accumulation tendency in the epidermis plus dermis sites (165.20 +/- 4.12 ng cm(-2)).