Grape-Seed Polyphenols Inhibit Aaa In Mice Via Regulation Of Macrophage Polarization

AIM: Abdominal aortic aneurysm (AAA) is characterized by macrophage polarization, and at present, no drug therapy is available. Although grape-seed polyphenols (GSP) showed an anti-AAA effect, the role of GSP in the phenotype shift in macrophages remains unknown.METHOD: The main phenolic compounds in GSP were determined by LC-MS. Male C57BL/6 mice were divided into four equal groups, namely sham group, CaPO4 group, CaPO4+GSP low-dose group, and CaPO4+GSP high-dose group. GSP was administered intragastrically after CaPO4 application. Molecular expressions were histologically evaluated and analyzed by various staining assays and FACS.RESULTS: GSP administration inhibited CaPO4-induced AAA formation, which correlated with a decrease in macrophage infiltration and retainment of vascular smooth muscle layer as compared to those in the CaPO4 group. FACS assay showed that the GSP administration dose-dependently decreased the CD54 expression (low-dose group: 11.4 +/- 2.1 % and high-dose group: 4.8 +/- 1.4 % vs 23.2 +/- 3.6 %; p < 0.05 and p < 0.01, respectively) and increased the CD206 expression of F4/80-positive cells in GSP-administered groups as compared with that in CaPO4-injured aortas in the CaPO4 group (low-dose group: 22.4 +/- 3.3 % and highdose group: 26.7 +/- 4.2 % vs 8.1 +/- 1.3 %; p < 0.05 and p < 0.01, respectively).CONCLUSIONS: GSP could be a pharmacologically potent agent in the treatment of AAA (Tab. 1, Fig. 3, Ref. 20). Text in PDF www.elis.sk