Kinetics Of Muscle Carnosine Decay After Beta-Alanine Supplementation: A 16-Wk Washout Study

PurposeThis study aimed to describe the kinetics of carnosine washout in human skeletal muscle over 16 wk. MethodsCarnosine washout kinetics were studied in 15 young, physically active omnivorous men randomly assigned to take 6.4 g center dot d(-1) of beta-alanine (n = 11) or placebo (n = 4) for 8 wk. Muscle carnosine content (M-Carn) was determined before (PRE), immediately after (POST), and 4, 8, 12, and 16 wk after supplementation. High-intensity exercise tests were performed at these same time points. Linear and exponential models were fitted to the washout data, and the leave-one-out method was used to select the model with the best fit for M-Carn decay data. Repeated-measures correlation analysis was used to assess the association between changes in M-Carn and changes in performance. ResultsM-Carn increased from PRE to POST in the beta-alanine group only (+91.1% +/- 29.1%; placebo, +0.04% +/- 10.1%; P < 0.0001). M-Carn started to decrease after cessation of beta-alanine supplementation and continued to decrease until week 16 (POST4, +59% +/- 40%; POST8, +35% +/- 39%; POST12, +18% +/- 32%; POST16, -3% +/- 24% of PRE M-Carn). From week 12 onward, M-Carn was no longer statistically different from PRE. Both linear and exponential models displayed very similar fit and could be used to describe carnosine washout, although the linear model presented a slightly better fit. The decay in M-Carn was mirrored by a similar decay in high-intensity exercise tolerance; M-Carn was moderately and significantly correlated with total mechanical work done (r = 0.505; P = 0.032) and time to exhaustion (r = 0.72; P < 0.001). ConclusionsCarnosine washout takes 12-16 wk to complete, and it can be described either by linear or exponential curves. Changes in M-Carn seem to be mirrored by changes in high-intensity exercise tolerance. This information can be used to optimize beta-alanine supplementation strategies.