Species and sex differences in eye morphometry and visual responsivity of two crepuscular sweat bee species (Megalopta spp., Hymenoptera: Halictidae)

Visually dependent dim-light foraging has evolved repeatedly, broadening the ecological niches of some species. Many dim-light foraging lineages evolved from diurnal ancestors, requiring immense visual sensitivity increases to compensate for light levels a billion times dimmer than daylight. Some taxa, such as bees, are anatomically constrained by apposition compound eyes, which function well in daylight but not in starlight. Even with this constraint, the bee genus Megalopta has incredibly sensitive eyes, foraging in light levels up to nine orders of magnitude dimmer than diurnal relatives. Despite many behavioural studies, variation in visual sensitivity and eye morphometry has not been investigated within and across Megalopta species. Here we quantify external eye morphology (corneal area and facet size) for sympatric species of Megalopta, M. genalis and M. amoena, which forage during twilight. We use electroretinograms to show that males, despite being smaller than females, have equivalent visual sensitivity and increased retinal responsivity. Although males have relatively larger eyes compared with females, corneal area and facet size were not correlated with retinal responsivity, suggesting that males have additional non-morphological adaptations to increase retinal responsiveness. These findings provide the foundation for future work into the neural and physiological mechanisms that interface with morphology to influence visual sensitivity, with implications for understanding niche exploitation.