Prediction of SARS coronavirus main protease by artificial Neural Network

SARS (Severe Acute Respiratory Syndrome) corona virus has hugely affected humans for more than ten years. Virus' RNA replicase gene is surrounding the polyprotein la and lab, and the sequence of the polyproteins contains functional proteins, which is an important factor of replication. The experiment was performed based on the "distorted key theory" in order to prevent SARS corona virus from performing replication by inactivating the main protease (also called CoV Mpro) of the virus. After the experiment, Neural Network (NN) was utilized in order to reanalyze the results of polypeptides in the virus. This approach by NN distinguished the fixed patterns in the sequence of cleavage site successfully, and improved the comprehension of the protease structure. The method of preventing the virus replication using competitive inhibitor could be found by analyzing these patterns.