Small-scale GMP production of plasmid DNA using a simplified and fully disposable production method

•Production process requires no specialized production equipment and no organic solvents•This manufacturing process yields sufficient pDNA for most phase I/II applications•pDNA complied to specifications, showing high purity measured by UV and AGE analyses•pDNA exhibits consistently high homogeneity in supercoiled plasmid topology•All executed runs were successful, vouching reproducibility of this GMP-compliant method