Human Cathepsin X/Z Is A Biologically Active Homodimer
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS(2021)
摘要
Human cathepsin X belongs to the cathepsin family of 11 lysosomal cysteine proteases. We expressed recombinant procathepsin X in Pichia pastoris in vitro and cleaved it into its active mature form using aspartic cathepsin E. We found, using size exclusion chromatography, X-ray crystallography, and small-angle X-ray scattering, that cathepsin X is a biologically active homodimer with a molecular weight of similar to 53 kDa. The novel finding that cathepsin X is a dimeric protein opens new horizons in the understanding of its function and the underlying pathophysiological mechanisms of various diseases including neurodegenerative disorders in humans.
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关键词
Cathepsin X, Cathepsin Z, Homodimer, Procathepsin X, Protease, Small-angle X-ray scattering
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