Immobilization of Invertase on Chitosan and Its Application to Honey Treatment

The optimal conditions for immobilized enzyme invertase on chitosan were studied. Beside that, the aim of the present work was to find out if the processing with chitosan invertase can affect some of the main honey quality paramenters - reductive sugar (RS), colour and antioxidant activity. RS content were analyzed by DNS method, colour parameters (L*, a*, b*) were established in the CIE system and antioxidant activity were analyzed by DPPH method. The results showed that the immobilized conditions were as follows: ratio chitosan/invertase 7/1 (w/w), invertase 0.8%, glutaraldehyde 4%, 50 degrees C at 60 minutes, pH 4.5. After the treatments, there was significant (P<0.05) difference on RS (58.38% for melaleuca honey, 15.11% for dimocarpus longan honey) and colour (Delta E=3.84 for melaleuca honey and Delta E =2.76 for dimocarpus longan honey). Moreover, there was no significant (P<0.05) difference on antioxidant activity.