Preparation of Amine-modified Poly (gamma-benzyl-L-glutamate) Guided Bone Regeneration Film

Guided bone regeneration film plays an important role in bone defect reconstruction. In this paper, poly (gamma-benzyl-L-glutamate) (PBLG) was used as raw materials to construct dense films and porous films by solvent casting and particle leaching. And the bilayers guided bone regeneration membrane was constructed by ethanolamine amine membrane modification on the surface. The effect of different modification time on the hydrophilicity and mechanical properties of the films was investigated. The results show that with the increase of the molecular weight of PBLG, the mechanical strength of the film increases and the degradation rate is getting slower. Prolonging the time of amine modification can improve the hydrophilicity of the film and the degradation rate both in vivo and in vitro. Through cell experiments, it was found that the dense layer could effectively block the invasion of fibroblasts, and the porous layer can support adherence and spread of cells. Surface-modified PBLG-based materials were demonstrated to be useful for repair of bone defects in vivo by in vitro bioactivity evaluation. The guided bone regeneration membrane constructed has good mechanical properties, degradation performance, and a certain degree of fit with the tissue. And it could effectively inhibit the infiltration of fibroblasts. The membrane has potential application value.