1259 EFFECTS OF OESTROGEN DEFICIENCY IN PLASMATIC PARAMETERS AND HEPATIC MITOCHONDRIAL FUNCTION

POSTERSto partial hepatectomy (70%) under 60 min of ischemia (PH+I/R).Retinol levels were measured and altered pharmacologically and their effects on hepatic damage and regeneration were studied after reperfusion.Retinol levels in steatotic livers from sham group were significantly higher than the levels in non-steatotic livers, indicating that the presence of fatty infiltration in and of itself induce changes in retinol metabolism.Thus, the effects of retinol were dependent on the type of liver.In non-steatotic livers, retinol levels in animals subjected to PH+I/R were similar to those of the Sham group.However, retinol levels in in steatotic livers undergoing PH+I/R were reduced in comparison with the Sham group.The administration of retinol increased hepatic injury and impaired liver regeneration in non-steatotic livers.In steatotic livers, retinol reduced damage and improved regeneration after surgery.These benefits of retinol were associated with reduced hepatocellular fat accumulation.Retinol did not modify oxidative stress or PPARa in steatotic livers, but did increase PPARg.Retinol actions could be mediated throught PPARg since inhibition of this latter signaling pathway abolished retinol benefits on regeneration and damage.In contrast to the key role of PPARg in these actions of retinol, the effects of retinol on lipid accumulation were PPARg-independent.Thus, in terms of clinical applications, retinol pre-treatment might open new avenues for liver surgery that specifically benefit the steatotic liver.