HLA-DRB1*0301 and HLA-DRB1*0401 Modify the Presentation and Outcome in Autoimmune Hepatitis Type-1

acute liver injury, the mice were pre-injected intravenously with 15mg/kg ROCK inhibitor, fasudil, fifteen minuetes ahead of Con A injection.Serum and liver tissue from individual mouse was collected.Serum alanine aminotransferase (ALT), aspartate animotransferase(AST) activities were measured.Inflammatory gene expression was determinated by real-time quantitative-RT-PCR.Liver inflammation was analyzed with H&E staining.F4/80,the marker of hepatic macrophages was detected by immunohistochemical staining.Hepatic CD4+T cells were isolated from liver tissue and followed by flow cytometry assay.Results: In the acute liver injury mice model induced by Con A, the ALT and AST level statistically decreased from 2356.57±1247.30U/L and 1972.00±707.84U/L in the Con A group to 221.71± 250.45U/L and 370.29±335.15U/L in the ConA/Fasudil group.Con A induced liver inflammatory gene expression (Tnfα, IFNγ, Cxcl10) and the RhoA/ROCK signaling activation, whereas intravenous pre-injection with ROCK inhibitor, fasudil abolished these phenotypes.Liver inflammation, the activation and proliferation of hepatic macrophages and CD4+ T cells were also suppressed by ROCK inhibitor.Conclusions