A multi-country field validation of the FluChip-8G Insight Assay

•This is a multi-country field validation of the FluChip-8G Insight, an assay which detects & characterizes seasonal & non-seasonal influenza.•Sensitivity and specificity for influenza A + B was 86 % and 100%, respectively. PPV was 100% (95% CI 99–100) and NPV was 73% (95% CI 68–78).•Sensitivity across all influenza subtypes was 100% for specimens with <20 and 20–25 Ct values, respectively.•As Ct values increased, sensitivity across all influenza subtypes decreased significantly(p < 0.001) for specimens with Ct values ≥32.•The assay showed good precision & reproducibility with robust identification of influenza with Ct values < 32 and in the absence of co-infection