Left Ventricular Diastolic Dysfunction in A Model of Obesity Induced by A High Carbohydrate Diet

Aim Evaluate the effect of diet obesity‐induced rich CHO on left ventricular diastolic function. Methods 16 male Wistar rats were assigned to receive water plus standard diet (group C, n = 8) and Ob diet with water plus 25% sucrose (Ob group, n = 8). Weekly body weight (BW) of the animals was assessed and, after 30 weeks of experiment, adiposity índex (AI). The following cardiac structures were measured: diastolic diameter (LVDD) and systolic (LVSD) LV; diastolic and systolic thickness posterior wall of the left ventricle (LVPWD; LVPWS) and interventricular septum (LVISD; LVISS); diameter of the aorta (DA), left atrium (LA) and the ratio (DA/LA); The relative thickness of the LV (ERVE); LV mass (LVM); MVE index (LVMI) was calculated by normalizing to body weight estimated LV mass. The LV systolic function was assessed by percentage of endocardial shortening (Δ% endo); The LV diastolic function was evaluated by the following indices: peak velocity of early diastolic filling (E wave); peak velocity of late diastolic filling (A wave); ratio between the E and A waves (E/A); deceleration time of E wave (DTE); isovolumetric relaxation time in absolute values (IRT) and normalized for heart rate (IRTn = IRT/R‐R 0,5). The study was supplemented by evaluation by tissue Doppler early diastolic (E′) and late (A′) of the mitral annulus (arithmetic average travel speeds of lateral and septal walls), and the ratio the waves (E/E′ and E′/A′). Results The ob group showed significantly higher weight (C = 493,008 ± 50,775, Ob = 562,95 ± 53,69; p < 0,05) and also higher adiposity index (C = 4.79 ± 0.73, Ob = 8.68 ± 1.76; p < 0,05). Results of the cardiac evaluation at 30 weeks are described in Table 1 . Conclusion Consumption of carbohydrate‐rich diets promotes left ventricular diastolic dysfunction associated with obesity at 30 weeks. Results of the cardiac evaluation performed at the end of 30 weeks. GRUPS Variables C Ob P BW (g) 493.008 ± 50.775 562.95 ± 53.69 0.018 LVDD (mm) 7.206 ± 0.201 6.705 ± 0.560 0.031 LVSD (mm) 3.116 ± 0.190 3.163 ± 0.431 0.785 LVPWD (mm) 1.626 ± 0.190 1.723 ± 0.226 0.373 LVPWS (mm) 3.039 ± 0.314 3.355 ± 0.213 0.033 LVISD(mm) 1.659 ± 0.194 1.864 ± 0.308 0.133 LVISS (mm) 3.355 ± 0.213 3.578 ± 0.193 0.045 DA (mm) 3.736 ± 0.129 3.993 ± 0.135 0.001 LA (mm) 4.756 ± 0.129 5.173 ± 0.383 0.011 LA/DA 1.274 ± 0.059 1.297 ± 0.107 0.609 LVDD/BW 14.755 ± 1.604 11.989 ± 1.304 0.002 LA/BW 9.740 ± 1.087 9.243 ± 0.897 0.335 LVM 0.817 ± 0.150 0.823 ± 0.152 0.937 LVMI 1.669 ± 0.318 1.475 ± 0.312 0.240 ERVE 0.451 ± 0.050 0.519 ± 0.095 0.098 CF (bpm) 234.875 ± 39.364 295.500 ± 26.966 0.002 Cardiac Output 81.181 ± 18.120 81.834 ± 28.74 0.957 % endo 56.743 ± 2.615 52.397 ± 8.591 0.190 E wave 68.350 ± 5.258 73.138 ± 3.312 0.046 A wave 40.675 ± 3.656 45.725 ± 6.040 0.062 E/A 1.684 ± 0.088 1.617 ± 0.163 0.327 IRT‐ms 21.125 ± 2.232 23.250 ± 3.012 0.131 DTE‐ms 47.250 ± 3.059 50.625 ± 2.722 0.035 R‐R (seg) 0.261 ± 0.042 0.205 ± 0.019 0.003 IRT/R‐R 41.696 ± 5.773 51.420 ± 5.826 0.004 Ejection fraction 0.918 ± 0.015 0.883 ± 0.063 0.142 E′ lateral 5.588 ± 0.412 5.225 ± 0.776 0.262 A′ lateral 3.550 ± 0.493 4.050 ± 0.578 0.083 E′ septal 5.850 ± 0.404 5.788 ± 0.906 0.861 A′ septal 3.950 ± 0.946 4.863 ± 0.691 0.044 E′ 5.791 ± 0.383 5.506 ± 0.735 0.479 A′ 3.750 ± 0.410 4.456 ± 0.540 0.010 E/E′ 12.016 ± 1.397 13.492 ± 1.867 0.093 E′/A′ 1.535 ± 0.145 1.260 ± 0.253 0.018