Tnf-Alpha Detection Using Gold Nanoparticles As A Surface-Enhanced Raman Spectroscopy Substrate
NANOMEDICINE(2020)
摘要
Background: TNF-alpha is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-alpha in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-alpha samples, combined with AuNPs, with decreasing concentrations of TNF-alpha. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-alpha/AuNPs (similar to 9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-alpha concentrations as low as 0.125 pg/ml. Conclusion: TNF-alpha signal at physiological concentrations was determined with SERS.
更多查看译文
关键词
biomarkers, cytokines, gold nanoparticles, SERS, TNF-α
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要