Effect of salts on size and morphology of extruded dimethyldioctadecylammonium bromide or chloride vesicle for polymeric nanocapsules synthesis via templating emulsion polymerization

In preparing polymer capsules by vesicle templated emulsion polymerization, the initial size and morphology of the biomimetic vesicle template dictate the final size and morphology of the capsules. The presence of salts (NaCl, NaBr and LiCl) influences the size, dispersity (PDI) and morphology of dimethyldioctadecylammonium bromide or chloride (DODAX, X  = Br− or Cl−) vesicles, prepared via membrane extrusion. DODAX vesicles in pure water exhibit broad size distributions with PDI of 0.5 and 0.3 for DODAB and DODAC, respectively. Addition of salts in water before (pre-addition) and after (post-addition) extrusion reduces the size and PDI of the vesicles significantly and results in various morphology investigated with cryo-TEM. It is observed that at low salt concentration (≤0.5 mM) in pre-addition, DODAX exists as a nice quasi spherical unilamellar vesicle, suitable for vesicle templated polymerization whereas in post-addition of salt at any concentration, the morphology is dominated by structures not suitable for templating application. The information obtained here is crucial for vesicle templated emulsion polymerization and it will be shown that there is a relationship between vesicle template morphology and final polymer capsule morphology.