Determination and Pharmacokinetic Study of Gefitinib in Rat Plasma by a Simple UPLC-MS/MS Method
LATIN AMERICAN JOURNAL OF PHARMACY(2015)
摘要
In this study, a simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method is described for determination of gefitinib in rat plasma samples using pirfenidone as the internal standard (IS) from pharmacokinetic assays. Sample preparation was accomplished through a simple protein precipitation with acetonitrile, and chromatographic separation was performed on an Acquity BEH C18 column (2.1 mm x 50 mm, 1.7 mu m) with gradient profile at a flow of 0.4 mL/min. The linearity of this method was found to be within the concentration range of 10-2000 ng/mL for gefitinib in rat plasma. Only 3.0 min was needed for an analytical run. The method was applied to a pharmacokinetic study after oral administration of 25 mg/kg gefitinib in rats.
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关键词
gefitinib,pharmacokinetic,rat plasma,UPLC-MS/MS
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