Carboxyl Methyltransferases: Natural Functions And Potential Applications In Industrial Biotechnology

The use of methyltransferases (MTs) in industrial biotechnology to replace toxic alkylating agents is of increasing interest. Carboxyl MTs (CMTs) are a subgroup of MTs that methylate the hydroxyl oxygen of carboxylic acids. Research initially focussed on their natural functions in protein regulation and production of volatile methyl esters in plants. In this review we highlight this potentially valuable group of enzymes that show promise for formation of a wide range of structurally diverse methyl esters from the parent acids under aqueous conditions. CMTs have been used to generate intermediates for biofuels, bioplastics and pharmaceuticals. These biocatalysts could also be integrated into cascades with other enzymes such as acyltransferases that function under aqueous conditions. Recent approaches for regenerating the required cofactorS-adenosylmethionine (SAM) are discussed includingin vitrorecycling, improvement inin vivoproduction and the use of more stable analogues. Advances in these areas will further improve the potential to use carboxyl MTs in industrial biotechnology.