Effects of obstacles and flow velocity on locomotory behavior in juvenile, silver carp, Hypophthalmichthys molitrix

We currently have only a minimal understanding of energy-saving strategies of fish in unsteady flows. In this study, we found that obstacle shape can affect swimming ability of fish: our results suggested that a half cylinder improved critical swimming speed of silver carp (Hypophthalmichthys molitrix) compared with swimming downstream of a full cylinder, square tube or for free-flow conditions. We also discovered how silver carp alter their locomotory behavior in response to turbulent flow caused by varying flow velocities around obstacles. At a flow velocity of 1 BL/s (body length per second), the fish spent similar percentages of time between holding station and moving forward behind the half cylinder: their motions resembled freestream swimming behavior. The greatest percentage of time spent downstream of the half cylinder occurred at a flow velocity of 5 BL/s. Fish were unstable and displayed irregular body motions with large lateral displacement at 5 BL/s. However, the fish held station for a long time and moved forward steadily behind the half cylinder at 3 BL/s. In holding station behind the half cylinder, fish maintained constant tail-beat frequency and tail- beat amplitude when flow velocity was increased from 1 to 3 BL/s. In moving forward behind the half cylinder, fish increased tail-beat frequency and tail-beat amplitude while maintaining constant ground swimming speed, swimming acceleration and ground stride when flow velocity was increased from 3 to 5 BL/s. Moreover, fish positioned their snouts in a small area approximately 1-3 BL downstream from the half cylinder at 3 BL/s. These behaviors support the hypothesis that turbulence behind a half cylinder was a suitable location for fish to conserve energy at 3 BL/s. Our results indicate that the combined effect of obstacles and flow velocity influence fish locomotory behavior, and some combinations may be beneficial to fish energy expenditure swimming in unsteady flow.