Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus Against Acetic Acid-Induced Inflammation in the Mouse Colon

Probiotic bacteria with anti-inflammatory properties have the potential to be of therapeutic benefit in inflammatory bowel diseases.The present study was designed to evaluate the effect of feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus on acetic acid-induced inflammation in mouse colon.Inflammatory model that mimics various features of IBDs was induced by a single application of 100µl of 4.5% acetic acid in Swiss Albino mice.Mice were pretreated orally by 200µl yogurt containing both L. acidophilus and L. bulgaricus for 3 days before induction of inflammation and 200µl yogurt was given orally for a period of 7 days after acetic-acid induction.The body weight, food and water intakes, serum biomarkers, macroscopic and histopathological studies of colon tissues were performed to evaluate the anti-inflammatory effect.Combined administration of both strains prevented the damages of villous and crypts in colon epithelial cells and thus provides unique mucosal protective effects in experimental colitis.In conclusion, feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus prevented or ameliorated the inflammatory conditions that can be beneficial to prevent or lower risks of IBDs and its complications.