Development and evaluation of reverse transcription loop-mediated isothermal amplification for rapid and real-time detection of Kyasanur forest disease virus

Jyoti S. Kumar,Pragya D. Yadav, Anita M. Shete,Triparna Majumdar, Savita Patil,Paban Kumar Dash

International Journal of Infectious Diseases（2021）

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摘要

•LAMP technology is isothermal and can be performed in dry bath.•LAMP technology is simple, rapid, highly sensitive and cost effective.•It can be use as point of care diagnostics.•Results can be obtained within 1h and interpreted by naked eye visualization.

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关键词

KFD,Molecular diagnosis,Gene,Isothermal,RT-LAMP

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