The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins

Kristina V Tugaeva,Dorothy E D P Hawkins,Jake L R Smith,Oliver W Bayfield,De-Sheng Ker,Andrey A Sysoev,Oleg I Klychnikov,Alfred A Antson,Nikolai N Sluchanko

Journal of Molecular Biology（2021）

引用 49|浏览6

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摘要

•SARS-CoV-2 nucleocapsid protein (N) binds to all seven human 14-3-3 isoforms.•This association with 14-3-3 strictly depends on phosphorylation of N.•The two proteins interact in 2:2 stoichiometry and with the KD in a μM range.•Affinity of interaction depends on the specific 14-3-3 isoform.•Conserved Ser197-phosphopeptide of N is critical for the interaction.

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关键词

host-pathogen interactions,protein–protein complex,phosphorylation,nucleocytoplasmic shuttling,stoichiometry

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