Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models

Efficient isolation, characterization, and culture of endometrial epithelial cells and stromal fibroblasts from calf uteri collected at the slaughterhouse is key to develop useful 3D culture tissue models to investigate uterine physiology and pathology without the need of performing invasive procedures to recover tissue samples. Here we provide a detail methodology that gives consistently pure and viable populations of distinct primary bovine endometrial cells.