Identification Of Aldehyde Oxidase 3 As A Binding Protein For Squid Ink Polysaccharides Using Magnetic Nanoparticles
RSC ADVANCES(2021)
摘要
To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis-Menten constant (K-m) was detected from a Lineweaver-Burk double reciprocal plot to assess effect of SIP on activity of aldehyde oxidase (AOX). Results showed that three proteins, AOX-3, regucalcin (RGN) and alpha 1-antitrypsin (A1AT3) were separated from mouse liver by magnetic nanoparticles conjugated with SIP. Contents of AOX-3 were much more than RGN and A1AT3. SIP (0.5 mg mL(-1)) reduced K-m value of aldehyde oxidase of mouse liver from 91.79 mu mol L-1 to 43.70 mu mol L-1.
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