Analysis Of K-Atp Channels Opening Probability Of Hippocampus Cells Treated With Kainic Acid

Background: Kainic acid (KA)-induced seizures may be a valuable tool in the assessment of anti-epileptic drug efficacy in complex partial seizures. This study investigated the effects of KA on ATP-sensitive K+ (K-ATP) channels opening probability (NPo), which plays a crucial role in neuronal activities.Methods: For the optimisation and validation protocol, beta-cells were plated onto 35 mm plastic petri dishes and maintained in RPMI-164o media supplemented with to mM glucose, to% FCS and 25 ntM of N-2-hydroxyethylpiperazine-N-ethanesulfonic acid (HEPES). The treatment effects of to mM glucose and 3o mu M fluoxetine on K-ATP channels NPo of beta-cells were assessed via cell-attached patch-clamp recordings. For hippocampus cell experiments, hippocampi were harvested from day 17 of maternal Lister-hooded rat foetus, and then transferred to a Ca-2(+) and Mg2+-free HEPES-buffered Hank's salt solution (HHSS). The dissociated cells were cultured and plated onto a 25 mm round cover glasses coated with poly-d-lysine (0.1 mg/mL) in a petri dish. The K-ATP channels NPo of hippocampus cells when perfused with 1 mM and 10 mM of KA were determined.Results: NPo of 13-cells showed significant decreasing patterns (P < 0.001) when treated with in mM glucose 0.048 (0.027) as well as 30 mu M fluoxetine 0.190 (0.141) as compared to basal counterpart. In hippocampus cell experiment, a significant increase (P < 0.001) in mean NPo 2.148 (0.175) of neurons when applied with 1 mM of KA as compared to basal was observed.Conclusion: The two concentrations of KA used in the study exerted contrasting effects toward the mean of NPo. It is hypothesised that KA at lower concentration (1 mM) opens more K-ATP channels, leading to hyperpolarisation of the neurons, which may prevent neuronal hyper excitability. No effect was shown in to mM KA treatment, suggesting that only lower than 10 mM KA produced significant changes in K-ATP channels. This implies further validation of KA concentration to be used in the future.