Protein A of Staphylococcus aureus strain NCTC8325 interacted with heparin
ARCHIVES OF MICROBIOLOGY(2021)
摘要
Heparin, known for its anticoagulant activity, is commonly used as the coatings of medical devices. The attaching of Staphylococcus aureus , a prominent human and animal pathogen, to the heparin coatings usually leads to catheter-related bloodstream infections. Hence, the study of the interaction between heparin and S. aureus surface proteins is desired. Here, we found that protein A (SpA) of S. aureus was a heparin-binding protein, contributing to the interaction between S. aureus and heparin. The cell-wall-anchored SpA was one of the most critical S. aureus virulence factors with a lysin-like motif (LysM). When SpA was mutated to remove the LysM motif, the heparin-binding capability of SpA dropped 50%. The in-frame deletion of spa also reduced the heparin-binding capability of S. aureu s. There was 1.3-fold more of heparin bound to wild type S. aureu s than the Δ spa::Em strain. These results would help understand the host–microbe interaction and the infection by S. aureus.
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关键词
Heparin, SpA, S, aureus, Infection
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