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SSR分子标记技术在汉青一号萝卜种子纯度鉴定中的应用

Xiao-kang ZHANG,Yun-feng ZHU,Yu-xia CHEN,Chang-cheng XU,Xue-li ZHANG,Qiu-fang XIONG

Hubei Agricultural Sciences（2016）

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Abstract
选用120对引物在汉青一号萝卜(Raphanus sativus L.cv.HanQing NO.1)亲本间进行PCR扩增,从中筛选出3对引物10、30、63,在双亲本间能够扩增出差异互补的条带,利用3对引物对汉青一号萝卜杂种1代群体进行纯度鉴定.鉴定结果纯度为95.1％,与同批次种子的田间鉴定结果96.0％相近,说明应用SSR分子标记技术实施萝卜杂种1代种子的纯度鉴定是可行并可靠的.
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