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一起羊黑疫的病原分离诊断及毒株产毒性能分析

Chinese Qighai Journal of Animal and Veterinary Sciences(2019)

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Abstract
利用厌氧菌实验室常规检测方法从一例送检病死羊肾脏培养物中分离到一株梭杆菌.通过形态学观察、动物实验、血清中和及胰酶破坏试验,确定分离病原菌为B型诺维氏梭菌.用自行设计的诺维氏梭菌α毒素特异性引物对纯培养物进行PCR扩增检测,PCR扩增得到大小为530bp的目的条带,与常规诊断结果一致;对分离的野生毒株的产毒能力进行了测定分析,野毒分离株在自制的VF厌气肝汤中的产毒能力达4000 MLD/mL,证明该菌株具备生产羊黑疫疫苗用株的潜力.
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