Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function after Damage

Context: The early events regulating the remodeling program following skeletal muscle damage are poorly understood. Objective: The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-.B) signaling by nutritionally accelerating the recovery of muscle function following damage. Design, Setting, Participants, and Interventions: Healthy males and females consumed daily postexercise and prebed protein-polyphenol (PP; n = 9; 4 females) or isocaloric maltodextrin placebo (PLA; n = 9; 3 females) drinks (parallel design) 6 days before and 3 days after 300 unilateral eccentric contractions of the quadriceps during complete dietary control. Main Outcome Measures: Muscle function was assessed daily, and skeletal muscle biopsies were taken after 24, 27, and 36 hours for measurements of myoPS rates using deuterated water, and gene ontology and NF-.B signaling analysis using a quantitative reverse transcription PCR (RT-qPCR) gene array. Results: Eccentric contractions impaired muscle function for 48 hours in PLA intervention, but just for 24 hours in PP intervention (P = 0.047). Eccentric quadricep contractions increased myoPS compared with the control leg during postexercise (24-27 hours; 0.14 +/- 0.01 vs 0.11 +/- 0.01%center dot h-1, respectively; P = 0.075) and overnight periods (27-36 hours; 0.10 +/- 0.01 vs 0.07 +/- 0.01%center dot h-1, respectively; P = 0.020), but was not further increased by PP drinks (P > 0.05). Protein-polyphenol drinks decreased postexercise and overnight muscle IL1R1 (PLA = 2.8 +/- 0.4, PP = 1.1 +/- 0.4 and PLA = 1.9 +/- 0.4, PP = 0.3 +/- 0.4 log 2 foldchange, respectively) and IL1RL1 (PLA = 4.9 +/- 0.7, PP = 1.6 +/- 0.8 and PLA = 3.7 +/- 0.6, PP = 0.7 +/- 0.7 log 2 fold-change, respectively) messenger RNA expression (P < 0.05) and downstream NF-.B signaling compared with PLA. Conclusion: Protein-polyphenol drink ingestion likely accelerates recovery of muscle function by attenuating inflammatory NF-.B transcriptional signaling, possibly to reduce aberrant tissue degradation rather than increase myoPS rates.