Direct Evidence Of Cytosolic Pngase Activity In Arabidopsis Thaliana: In Vitro Assay System For Plant Cpngase Activity
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY(2021)
摘要
Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.
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关键词
cytosolic PNGase, acidic PNGase, PNGase assay, free N-glycan, Arabidopsis thaliana
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