Analysis of T Cell Responses to Helicobacter pylori Infection

Analysis of human and mouse T-helper (Th) cell subset responses is key to understanding how the inflammatory response in the stomach is regulated, and links with pathology and disease outcomes. Here, we present methods for extracting cells from blood and tissue, and analyzing the balance of Th1, Th2, Th17, and regulatory T cell (Treg) subsets using flow cytometry and cytokine ELISA.