Quantitative And Qualitative Assessment Of Small Rna Preparations
SMALL NON-CODING RNAS, 2 EDITION: Methods and Protocols(2021)
摘要
Recent advances in high-throughput sequencing have shed new light on the diversity of small noncoding RNA (sncRNA) classes and their crucial roles in gene regulation and disease. One key step in sncRNA profiling consists in their quantification and assessment of their degradation extent. In this chapter, we will describe different gold standard methods used to achieve both purposes before using the sncRNAs in downstream applications.
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关键词
RNA quantification, UV spectroscopy, Fluorescence, Capillary electrophoresis, RNA integrity number
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