Analysis of mouse male germ cell-specific or -predominant Tex13 family genes encoding proteins with transcriptional repressor activity

Mammalian spermatogenesis is a highly organized process with successive mitotic, meiotic, and postmeiotic phases. This unique developmental process is characterized by the involvement of spermatogenic cell-specific genes. In this study, we identified and investigated testis expressed gene 13 ( Tex13 ) family genes, consisting of Tex13a, Tex13b, Tex13c1, and Tex13d , in mice. All of these genes were transcribed specifically or predominantly in male germ cells, and their transcription was developmentally regulated. Proteins encoded by the Tex13 genes were predicted to have a conserved domain of ~ 145 amino acids. Tex13a, Tex13c1 , and Tex13d encode additional C-terminal regions containing a short conserved sequence termed a zinc finger-RAN binding protein 2 (zf-RanBP2) or zf-RanBP2-like domain. As TEX13B reportedly has transcriptional repressor activity, we examined the effect of the TEX13 proteins on transcriptional regulation using a reporter assay. All of the TEX13 proteins exhibited transcriptional repressor activity. This activity was revealed to reside in the TEX13B-corresponding regions of TEX13A, TEX13C1, and TEX13D. Further, we found that the C-terminal regions of TEX13A, TEX13C1, and TEX13D also have inhibitory activities. These results suggest that male germ cell-specific or -predominant TEX13 proteins commonly function in transcriptional repression as transcription cofactors and/or RNA binding proteins.