Metal Ions Coordinated Immobilization Of Phenylalanine Dehydrogenase By Go-Pei With High Activity Recovery And Enhanced Stability

BACKGROUND: Dehydrogenases dependent on NAD(P)H are widely applied for biosynthesis of chiral chemicals. However, they are unstable and easy denaturated compared with hydrolases. A new strategy for dehydrogenases immobilization with the advantages of easy operation and high activity recovery should be developed. Biocompatible hybrid materials (GO-PEI-M) have been obtained by metal ions coordinated polyethylenimine (PEI) functionalized graphene oxide (GO).RESULTS: The immobilized enzyme recovery rates of GO-PEI-Mg (magnesium ion coordinated GO-PEI) and GO-PEI-Mn (manganese ion coordinated GO-PEI). In addition, the stability of GO-PEI-Mn-PheDH (Phenylalanine Dehydrogenase) and GO-PEI-Mg-PheDH was greatly improved, and it maintained 65% and 79% activity, respectively, after seven times reuse. In addition, the stability of GO-PEI-Mn-PheDH (Phenylalanine Dehydrogenase) and GO-PEI-Mg-PheDH was greatly improved. After seven times reuse, GO-PEI-Mn-PheDH and GO-PEI-Mn-PheDH and maintained 65% and 79% activity, respectively. An assembly mechanism was proposed from the perspectives of multi-level interactions, including hydrogen bonding, electrostatic forces, and coordinated bonds.CONCLUSION: GO-PEI-Metal ion hybrid materials offer a promising carrier for enzyme immobilization with the advantages of biocompatibility and relatively strong interaction. (c) 2020 Society of Chemical Industry