Forensic evaluation of two nucleic acid extraction systems and validation of a RT-qPCR protocol for identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs

Pedro A Barrio, Amparo Fernández-Rodríguez,Pablo Martín,Coro Fernández, Lourdes Fernández,Antonio Alonso

Forensic Science International（2021）

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摘要

•The INTCF has validated the molecular detection of SARS-CoV-2 for its forensic use.•Automated virus RNA extraction has greater relative efficiency than the manual one.•Molecular detection of SARS-CoV-2 with the kit tested is accurate and sensitive.

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关键词

Coronavirus Infectious Disease-19 (COVID-19),Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2),Quantitative reverse transcription PCR (RT-qPCR),Validation,Forensic microbiology,Forensic genetics

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