Distinct Repopulation Activity in Hu-Mice Between CB- and LPB-CD34+ Cells by Enrichment of Transcription Factors.
INTERNATIONAL JOURNAL OF STEM CELLS(2021)
摘要
BACKGROUND AND OBJECTIVES:Human CD34+ hematopoietic stem cells can reconstitute the human hematopoietic system when transplanted into immunocompromised mice after irradiation. Human leukapheresis peripheral blood (LPB)- and cord blood (CB)-derived CD34+ cells have a similar capacity to reconstitute myeloid lineage cells in a humanized mice (hu-mice) model. However, potent stem cells, such as CB-CD34+ cells, efficiently reconstitute the lymphoid system in vivo compared to LPB-CD34+ cells. Modeling the human hematolymphoid system is vital for studying immune cell crosstalk in human xenografted mice, with CB-CD34+ cells used as an optimized cell source because they are essential in reconstituting lymphoid lineage cells.
METHODS AND RESULTS:In this study, we established hu-mice that combined human characteristics with long-term survival and investigated the efficiency of the engraftment of lymphoid lineage cells derived from LPB- and CB-CD34+ cells in the bone marrow, spleen, and LPB. We found an overall increase in the transcriptional activity of lymphoid lineage genes in CB-CD34+ cells. Our results revealed that potent CB-CD34+ cells displaying a general upregulation of the expression of genes involved in lymphopoiesis could contribute to the hematolymphoid system in the humanized mice model with longevity.
CONCLUSIONS:Our data suggest that humanized mouse model by usage of CB-CD34+ cells displaying high expression of TFs for lymphoid lineage cells can contribute to study the immune response against lymphocytes.
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关键词
Humanized mice model, Hematolymphoid lineage cells, Cord blood CD34(+) cells, Transcription factor enrichment
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