Screening And Characterization Of A Novel Reversible 4-Hydroxyisophthalic Acid Decarboxylase From Cystobasidium Slooffiae Htk3

Owing to carboxylation activity, reversible decarboxylases can use CO2 as a C1-building block to produce useful carboxylic acids. Although many reversible decarboxylases can synthesize aromatic monocarboxylic acids, only a few reversible decarboxylases have been reported to date that catalyze the synthesis of aromatic dicarboxylic acids. In the present study, a reversible 4-hydroxyisophthalic acid decarboxylase was identified in Cystobasidium slooffiae HTK3. Furthermore, recombinant 4-hydroxyisophthalic acid decarboxylase was prepared, characterized, and used for 4-hydroxyisophthalic acid production from 4-hydroxybenzoic acid.[GRAPHICS]Reversible decarboxylase that can synthesize 4-hydroxyisophthalic acid obtained from an isolated strain Cystobasidium slooffiae HTK3.