Non-target bioanalytical eight-dimensional hyphenation including bioassay, heart-cut trapping, online desalting, orthogonal separations and mass spectrometry

•Robust non-target bioanalytical screening for bioactive compounds was developed.•Eight-dimensional hyphenation: NP-HPTLC–UV/Vis/FLD–EDA–RP-HPLC–PDA–MS•Analyte trapping and online desalting reduced the transfer of bioassay salts to MS.•Orthogonal separations improved the separation power for complex samples.•Proof-of-principle was successfully made for active compounds in 18 cinnamon samples.